Regenerative medicine, Regeneration, Tissue, Cell, Skeletal muscle, Research yon Shin "Skeletal Muscle Regeneration" Harvard Medical School
Maybe an u.s suffer from volumetric uh around 500 patients and you suffer from volumetric muscle loss and one of the interesting. I think in the description that you maybe mimic the muscle architecture and as well as you figuring out what be the growth parameters that you can. Maybe regenerate or maybe reinvent in your design. So if you can tell us about that, because i think it’s very interesting how you approach this problem and how you figure out the growth parameter in a human being yeah, so yeah that’s. Why i’m developing you know soft actuator to you know, help the patient who lost you know arm and then lack, and in this case like we need to give them. You know um, like a patient like a thick vascularized muscle tissue so but when we create, when i create a dic, basically right, muscle tissue. One of the major challenges like how to create a better network within the thick muscle tissue without pestle, which can provide oxygen and nutrient, then most of cell, might die in the tissue console even in vitro and vivo. Those is major challenges so to combine, but a muscle and vessel like different uh stiffness of the material. Then you need to combine both uh. You know two different material which is deep and you know stiff and hard and combined together, you need. We need to create a basically muscle tissue, so in this case i use like a bioprinting technique to easily integrate incorporate two different material using multimaterial printing technique, they’re also using the biomaterial.
This much biomaterial is good for the differentiation of the stem cell. Also, the maturation of the muscle or the formation of the vessel, so i have been studying for many different type of biomaterial to find which material is good for the muscle and the which material group for the vessel and we already defined, and they make two different. Uh, you know the material category, then they will pick the good material which can be uh printed, that and then uh and then we can create basically rise, the scaffold using bioprinting technique. Another point is for important point is like a cell source, so we can use many. We can try many different type of source, but when you implant this in vitro culture tissue, we have to minimize immune response from with of the patients, then also we need to improve the integration of this in vitro cultural muscle tissue into the injured area. Then now it’s, very promising a cell source is ips derived the muscle cell ips derived the stem cell, which can obtain from you know, human and skin skin tissue. So this eye pester can differentiate to the um, the muscle tissue, also vessel. Then, when we use this patient cell and combined with this advanced, biomaterial and micro fabrication technique features by printing, then we can create personalized, vascularized muscle tissue that it improved different. I believe this system can be improved, muscle and tissue regeneration for the patient that’s very interesting.
Indeed, and maybe ask a little question here about what first, what could be the limitation you face already in because when you speak about smart material, we try to combine different materials, different mechanical properties. Sometimes we have visible adhesion. Obviously the elimination happen and we try to combine life cells with smart material and if you can tell us what could be still limitation for you, yeah of course a lot of telling patients so because you know mostly efficient so biomaterial you know can make. Can you know, maintains a viability, but this means that the cells show their function right then, and then most important. We can mimic like a muscle contract it’s hard to make hard to you know mimic the function of the muscle means like our muscle is very strong, and then you can. You know it’s very if it has good mechanical property also, they show contraction. Behavior extremely. You know useful and efficient with low power, energy right, low power, then and then but um it’s, still a lot of challenges to mimic those function and efficiency with in vitro cultural tissue.